Activation of human epithelial lung a549 cells by the pollutant sodium sulfite: enhancement of neutrophil adhesion.

Air pollutant exposure may induce deterioration of respiratory health. Concentrations of air particles, ozone, nitrogen dioxide, sulfur dioxide, and sulfate are among the players involved in the initiation and/or exacerbation of lung diseases. We have previously documented that the pollutant sodium sulfite (Na(2)SO(3)) is a human neutrophil agonist. To date, there is no evidence in the literature that Na(2)SO(3) can activate epithelial lung cells. In the present study, we found that Na(2)SO(3) (0.01-10 mM) induces tyrosine phosphorylation events and interleukin-8 production in human epithelial lung A549 cells. In addition, we found that Na(2)SO(3) did not promote A549 cell apoptosis as assessed by the degradation of the cytoskeletal gelsolin protein and by FITC-annexin-V binding. Human neutrophil adhesion to Na(2)SO(3)-induced A549 cells was increased when compared with untreated A549 cells. As assessed by flow cytometry, cell surface expression of intercellular adhesion molecule (ICAM)-1, ICAM-3, and vascular cell adhesion molecule-1 (VCAM-1) on A549 cells was not affected by Na(2)SO(3). We conclude that Na(2)SO(3) can activate A549 cells. In addition, we conclude that neutrophil adhesion to Na(2)SO(3)-induced A549 cells is increased via an ICAM-1-, ICAM-3-, and VCAM-1-independent mechanism.